| 학회 | 한국화학공학회 |
| 학술대회 | 2015년 봄 (04/22 ~ 04/24, 제주 ICC) |
| 권호 | 21권 1호, p.404 |
| 발표분야 | 생물화공 |
| 제목 | Metabolic engineering of Corynebacterium glutamicum that produces succinate from levoglucosan |
| 초록 | Development of economically feasible technologies for biomass-derived sugars is still a challenge. The pyrolysis can be used to convert biomass into an energy-rich liquid (bio-oil). Pyrolysis of untreated biomass can produce bio-oil that contains up to 12% levoglucosan (1,6-Anhydro-beta-glucopyranose). In this work, we aim to engineer Corynebacterium glutamicum, a widely-known industrial bacterium for productions of amino acid and varieties of biochemical, to utilize levoglucosan as a sole carbon source. We selected several levoglucosan kinases (LGK) and cloned into a CoryneBrick vector for C. glutamicum. The strain harboring LGK from Lipomyces starkeyi can grow by utilization of levoglucosan as sole carbon. For the production of succinate, recombinant LGK was expressed in C. glutamicum BL1 strain, which is an engineered strain for succinate production. Comparable production has been achieved compare to the production from glucose. This work will broaden the possibility of utilization of biooil as potential feedstocks. This work was supported by the R&D Convergence Program of NST (National Research Council of Science &Technology). |
| 저자 | 우한민1, 김은미1, 엄영순1, 최준원2 |
| 소속 | 1한국과학기술(연), 2서울대 |
| 키워드 | 코리네박테리움; 레보글루코산; 숙신산 |
| 원문파일 | 초록 보기 |
