| 학회 |
한국화학공학회 |
| 학술대회 |
2014년 봄 (04/23 ~ 04/25, 창원컨벤션센터) |
| 권호 |
20권 1호, p.425 |
| 발표분야 |
생물화공 |
| 제목 |
Production of 2-Hydroxybutyrate containing polymer by Metabolic Engineered Escherichia coli |
| 초록 |
Polyhydroxyalkanoates(PHAs) are biodegradable and biocompatible polyesters. To enhance polymer properties, E. coli was metabolically engineered to synthesize PHAs containing 2-hydroxybutyrate (2HB) from glucose. For Heterologous metabolic pathway to supply 2-hydroxybutyrate from glucose was constructed via the citramalate pathway. Recombinant E. coli expressing the phaC1437, pct540, cimA3.7, and leuBCD genes together with the L. lactis II 1403 panE gene successfully produced PHAs consisting of 2HB, 3HB, and a small fraction of lactate by varying the 3HB concentration. [“This work was supported by the Technology Development Program to Solve Climate Changes from National Research Foundation of Korea (Development of systems metabolic engineering platform technologies for biorefineries; NRF-2012-C1AAA001-2012M1A2A2026556) and Intelligent Synthetic Biology Center (2011-0031963) of Korea through the Global Frontier Research Program of the Ministry of Education, Science and Technology (MEST).” ] |
| 저자 |
최소영1, 양정은1, 박시재2, 이승환2, 송봉근2, 이상엽1
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| 소속 |
1KAIST, 2화학(연) |
| 키워드 |
bioplastic; 2-hydroxybutyrate; metabolic engineering
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| E-Mail |
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| 원문파일 |
초록 보기 |
