| 학회 |
한국공업화학회 |
| 학술대회 |
2020년 가을 (10/28 ~ 10/30, 광주 김대중컨벤션센터(Kimdaejung Convention Center)) |
| 권호 |
24권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Efficient conversion of 3-hydroxypropionate from acetate in Escherichia coli |
| 초록 |
Acetate is a potential feedstock for microbial processes that produce diverse value-added chemicals. In this study, we produced 3-hydroxypropionic acid (3-HP) from acetate in engineered Escherichia coli. For efficient production of 3-HP, we initially introduced heterologous malonyl-CoA reductase gene from Chloroflexus aurantiacus. Then, the acetate assimilating pathway and glyoxylate shunt pathway were activated by overexpressing acetyl-CoA synthetase and deleting glyoxylate shunt pathway repressor. Because a key precursor malonyl-CoA is also consumed for fatty acid synthesis, we decreased carbon flux to fatty acid synthesis by adding cerulenin. Subsequently, we found that inhibiting fatty acid synthesis dramatically improved 3-HP production (3.00 g/L of 3-HP from 8.98 g/L of acetate). The results indicated that acetate can be used as a promising carbon source for microbial processes and that 3-HP can be produced from acetate with a high yield (44.6% of the theoretical maximum yield). |
| 저자 |
이지연, 차상학, 이지훈, 강채원, 이건민, 임현규, 정규열
|
| 소속 |
포항공과대 |
| 키워드 |
Metabolic engineering; acetate; 3-hydroxypropionate
|
| E-Mail |
|
