| 학회 |
한국공업화학회 |
| 학술대회 |
2009년 봄 (04/15 ~ 04/17, 호텔인터불고(대구)) |
| 권호 |
13권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Development and Evaluation of Efficient Recombinant Escherichia coli Strains for the Production of 3-Hydroxypropionic Acid from Glycerol |
| 초록 |
3-hydroxypropionic acid (3-HP) is a potential raw material for producing many industrial chemicals. Previously, we produced 3-HP from glycerol by recombinant E. coli SH254 expressing dhaB and aldH. In this study, efficiency of strain(s) was improved by expressing these genes in different IPTG inducible plasmids along with reactivase (GDR). In addition, by replacing aldH with KGSADH-I (E. coli SH-BGK1), exhibited a highest production of 3-HP at 2.8 g/L in shake-flask conditions, and 38.7 g 3-HP/L in bioreactor. Here, for the first time, we demonstrate the production of 3-HP at a highest titer from glycerol. |
| 저자 |
Chelladurai Rathnasingh, Subramanian Mohan Raj, Ji-Eun Jo, Woo-Chel Jung, 박성훈
|
| 소속 |
부산대 |
| 키워드 |
GDH; GDHR; 3-Hydroxypropionic acid; Glycerol; ALDH; KGSADH
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| E-Mail |
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