| 초록 |
Recently, many people are interested in therapy or diagonosis of diseases. Polydiacetylene liposomes have been used for gene, drug delivery and biosensor research. So we studied for gene delivery with 10,12-pentacosadiynoic acid(PCDA) derivatized polydiacetylene. We made a liposome that was conjugated with low molecular weight polyethyleneimine(PEI) on the 10,12-pentacosadiynoic acid liposome. We used to polymerization PCDA for in this study. PCDA-PEI liposome was confirmed by nuclear magnetic resonance (NMR) spectroscopy and Fourier Transform Infrared Spectrometer (FT-IR). Agarose gel electrophoresis and pico-green reagent assay were ensured to complex formation of the synthesized polymer and plasmid DNA and the size of liposome was measured by dynamic light scattering (DLS). Transfection efficiency and cytotoxicity was evaluated in HeLa and HEK293 cells. |
