| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 가을 (11/08 ~ 11/10, 부산 벡스코(BEXCO)) |
| 권호 |
21권 2호 |
| 발표분야 |
(생물공학) 생물산업의 미래를 위한 신기술: 분자로부터 시스템까지 |
| 제목 |
Metabolic Engineering of microorganisms for the production of 3-hydroxypropionic acid from glycerol |
| 초록 |
For every 9kg of biodiesel production, about 1kg of crude glycerol is generated as a byproduct. Glycerol is a good carbon source for the production of 3-hydroxypropionic acid (3-HP). Several engineered microbes such as Escherichia coli, Klebsiella pneumoniae and Pseudomonas denitrificans have been developed for the production of 3-HP by introducing an aldehyde dehydrogenase and a glycerol dehydratase. However, both the enzymes were seriously inactivated during the reaction. To minimize the inactivation of the enzymes, balanced expression of the genes was attempted by promoter engineering and unique dynamic promoters which are induced by 3-HP have been employed. The dynamic promoters could express the genes of the two enzymes continuously and maintained their activity until the end of fermentation. This talk summarizes our long efforts for the 3-HP production and discusses the potential and limitations of our work. Also, glycerol metabolisms affecting 3-HP production are discussed. |
| 저자 |
박성훈 |
| 소속 |
울산과학기술원 |
| 키워드 |
Metabolic Engineering; 3-hydroxypropionic acid
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| E-Mail |
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