| 초록 |
Nucleic acids, with their perfect binding capability, have been intensively investigated for their use as building blocks and structure-directing components in so-called “bottom-up” approach to generate self-assembled architectures with a precise control at the nanometer scale. Due to the well-defined nanostructure, DNA nanoarchitectures have various potential applications in chemistry, biology, electronics and so on. In this presentation, we report a new DNA probe working on the principle of the fluorescence change via programmed assembly to build a novel class of DNA nanoarchitecture. In our ODN-Pyene-ODN probe, the consecutive hybridizations between the probes and target oligonucleotides induce programmed core-shell (Pyrene-ODN) nanodot assembly to generate their excimer emission. This fluorescent probe (ODN-Pyrene-ODN) was shown to detect target oligonucleotides with high reliability via DNA core-shell nanodot architecture generating specific fluorescence modulation. |
