| 학회 |
한국화학공학회 |
| 학술대회 |
2012년 봄 (04/25 ~ 04/27, 제주 ICC) |
| 권호 |
18권 1호, p.1437 |
| 발표분야 |
Synthetic biology for sustainable chemical industry |
| 제목 |
Re-cycling cyclic peptides through peptidyl-tRNA drop-off |
| 초록 |
During translation, the escape of peptidyl-tRNA from the P-site, called peptidyl-tRNA drop-off happens, which is believed to be essential in all living forms. This "loss in translation" has been known for a long time, but our understanding on peptidyl-tRNA drop-off was limited. We found that the site of peptidyl-tRNA drop-off could be pinpointed in translation of histone H3 N-terminal tail, which can facilitate the studies on peptidyl-tRNA drop-off. Using Histone H3 tails as a model peptide, we here show that codon reprogramming method can be successfully utilized to help understanding peptidyl-tRNA drop-off, and some of preliminary results regarding the event are presented. We also show that a simple twist in idea that "utilizes" peptidyl-tRNA drop-off can be used to synthesize cyclic peptides and possibly proteins using an example of sunflower trypsin inhibitor-1. |
| 저자 |
DVN Sudheer Pamidimarri1, Hiroaki Suga2, 강택진3
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| 소속 |
1동국대, 2Dept. of Chemistry, 3School of Science |
| 키워드 |
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| E-Mail |
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| VOD |
VOD 보기 |
| 원문파일 |
초록 보기 |
