| 초록 |
Inclusion complexes (ICs) of hydrophobic oligopeptide by cationic β-cyclodextrins (CDs) containing histidine and amine moiety were prepared respectively. The internalization of different ICs prepared with different molar ratio (1:0, 7:3, 5:5, 3:7 and 0:1) between of histidine modified β-CD and amine modified β-CD was investigated. Flow cytometry and spectrofluorimetry analysis indicated that ICs exhibited significant cellular uptake compared with free oligopeptide drug. Cell viability study showed that both amine and histidine modified β-CD no significant cytotoxicity was observed. Mechanism of cell-penetrating and pharmacokinetics study show amine and histidine modified β-CDs with cell-penetrating properties were potential carriers for hydrophobic peptide drugs. |
