| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO)) |
| 권호 |
23권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Development of artificial naringenin riboswitches in vivo |
| 초록 |
Genetically encoded biosensors have been applied for high-throughput screening or dynamic regulation of biosynthetic pathways for enhancing the performance of microbial strains that produce valuable chemicals. Here, we developed artificial riboswitches that activate gene expression in response to naringenin. RNA aptamers for naringenin were developed using SELEX and cloned upstream of a dual selectable marker gene to construct a riboswitch library. Two in vivo selection routes were applied separately to the library by supplementing naringenin at two different concentrations during enrichments to modulate the operational ranges of the riboswitches. The selected riboswitches were responsive to naringenin and activated gene expression up to 2.91-fold. Operational ranges of the riboswitches were distinguished on the basis of their selection route. Additionally, the specificity of the riboswitches was assessed, and their applicability as dynamic regulators was confirmed. |
| 저자 |
이건민1, 장성호1, 장성연1, 강택진2, 이상협3, 정규열1
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| 소속 |
1포항공과대, 2동국대, 3대구가톨릭대 |
| 키워드 |
aptamer; flavonoid; naringenin; riboswitch; riboswitch tuning; sensor-regulator
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| E-Mail |
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