| 초록 |
Bacteria sensor was designed by employing boronic acid-conjugated carbon dot combined with dye as a quencher (CD-D), resulting fluorescence quenching of CD-D due to forster resonance energy transfer (FRET). Detection was depended on diol-diol interaction between boronic acid in CD-D and glucose in bacteria, which resulted the release of dye and recovery of CD fluorescence intensity. CD-D showed excellent performance on fluorescence-based bacteria detection with limit of detection to be 101 CFU/mL towards S. aureus and E. coli. In addition, the binding of CD-D sensor with bacteria was observed via electrochemical method, with more sensitivity compare to fluorescence approach (101 CFU/mL). Furthermore, the CD-D can be applied on real sample such as river water and showed good performance on bacteria sensing. Thus, this sensor offers simple and selective approach for bacteria detection in environmental matrix. |
