| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Effect of homologous overexpression of citrate synthase on glyoxylate pathway genes and acetate assimilation in Pseudomonas putida |
| 초록 |
Glyoxylate pathway plays important role in the assimilation of acetate for growth in various microorganisms including Pseudomonas putida. Once acetate is converted into acetyl-CoA, percentage of Acetyl-CoA funnelling between to TCA or glyoxylate cycle and PHA synthesis is not explored. In this study, we homologously overexpressed citrate synthase gene to divert acetyl-CoA to TCA/glyoxylate cycle. The effect of citrate synthase overexpression on other glyoxylate pathway genes was quantified using RT-PCR and acetate metabolization was compared between wild type and mutant P. putida. This study provide an potential application of bioconversion of abundant but refractory simple acetic acid into useful metabolites and chemicals which dependent on glyoxylate cycle. |
| 저자 |
mutyala sakuntala, 김중래
|
| 소속 |
부산대 |
| 키워드 |
Acetate assimilation; gene over expression; glyoxylate pathway
|
| E-Mail |
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