| 초록 |
Clostridium thermocellum is a thermophilic and cellulolytic anaerobe. It metabolizes cellulose to cellobiose and cellodextrin, which are then taken up by the cells and undergo glycolysis yielding H2, CO2, formate, lactate, acetate, and ethanol as mixed co-products. Hydrogen metabolism in C. thermocellum is catalyzed by hydrogenase enzymes, which can be classified as FeFe- or NiFe-type based on the metal content in its active site. Analysis of the sequenced genome of C. thermocellum reveal the presence of up to four hydrogenases, three of which belonging to the FeFe-type, one the NiFe-type.The bacterium is remarkably versatile in employing various enzymes, some of which are potentially novel, for hydrogen metabolism. Little is known as to their underlying physiological functions and their contribution in fermentative H2 production.More in-depth knowledge and biochemical information of the hydrogenases in C. thermocellum will help engineer the microbe for improved H2 production. |
