| 초록 |
Silica is in the spotlight of biotechnology as a supporting material because of its stability, biocompatibility, mechanical property, etc. Existing methods of silica synthesis are based on chemical process that requires high pressure, high temperature, high/low pH. These harsh conditions are improper for biological applications. In this study, we used SFP(silica forming peptide) which synthesize biosilica under ambient conditions. The objective of this work is to immobilize enzyme into silica particle mediated by SFP fused enzyme and to study the influence of encapsulated enzyme with biosilica. The model enzyme is formate dehydrogenase (FDH) which catalyzes the oxidation of formate to CO2 from Candida boidinii. New SFP derived from marine species was fused to C-terminus of FDH without loss of catalytic activity of it. FDH-SFP was auto-encapsulated with silica by mix with TMOS under aqueous ambient condition. Silica-immobilized FDH displayed an increase in enzyme stability and maintained 80% activity until 10 times recycling. We expect SFP-mediated biosilicification provides a new tool for efficient enzyme immobilization in industrial applications. |
