| 학회 | 한국화학공학회 |
| 학술대회 | 2017년 봄 (04/26 ~ 04/28, ICC 제주) |
| 권호 | 23권 1호, p.517 |
| 발표분야 | 생물화공 |
| 제목 | Direct evolution of enzyme for enhanced production of biopolymer in Escherichia coli |
| 초록 | Nowadays, researchers working on find out alternative of synthetic plastic because of oil exhaustion and increasing of concerning for environment. Petroleum based plastic cause serious problems to ocean, land and air by it’s hard to decomposed property. Bioplastic is one of strong candidate to replace the chemical plastics and it derived from renewable biomass sources. Biopolymers have biodegradable property in certain condition with bacteria, algae, molds and enzyme. P(3HB) is the most common and important biosynthesis PHA and produced well in various bacteria with PHA synthase. In this research, we performed the evolutionary engineering of PHA synthase by FACS for the enhanced production of PHA biopolymer in E. coli. PHA biopolymer can be stained by fluorescent probes (BODIPY, Nile Red etc), and the content of PHA synthesized in host can be quantified by flow cytometer. First, we optimized the staining condition with BODIPY to improve the correlation between intracellular PHA content and fluorescent intensity. Then, using the optimized staining method, we conducted the FACS-based high throughput screening of random library to isolate high-PHA producing clones. |
| 저자 | 이재형, 정기준 |
| 소속 | 한국과학기술원 |
| 키워드 | 생물화공 |
| 원문파일 | 초록 보기 |
