| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Efficient production of 3-hydroxypropionic acid from acetate in engineered Escherichia coli. |
| 초록 |
Acetate is a potential feedstock for microbial processes to produce diverse value-added chemicals. In this study, we produced 3-hydroxypropionic acid (3-HP) from acetate with engineered Escherichia coli. For the efficient conversion of acetate to 3-HP, we introduced heterologous malonyl-CoA reductase from Chloroflexus aurantiacus. Then, the acetate assimilating pathway and glyoxylate shunt pathway were activated by overexpressing acetyl-CoA synthetase and deleting glyoxylate shunt pathway repressor. Since key precursor malonyl-CoA is also consumed for fatty acid synthesis, we decreased carbon flux to fatty acid synthesis by adding cerulenin. Subsequently, we found that inhibiting fatty acid synthesis dramatically improved 3-HP production (3.00 g/L of 3-HP from 8.98 g/L of acetate). The results indicated that acetate can be used as a promising carbon source for microbial processes and that 3-HP can be produced from acetate with a high yield (44.6% of the theoretical maximum yield). |
| 저자 |
이지연1, 차상학1, 이지훈1, 강채원1, 이건민1, 임현규2, 정규열1
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| 소속 |
1포항공과대, 2UCSD |
| 키워드 |
Escherichia coli; Acetate; 3-hydroxypropionic acid
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| E-Mail |
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