| 초록 |
Microalgae have gained considerable attention due to their ability to produce economically valuable bio-products using CO2. However, various biological phenomena are still unknown. We investigated chemotaxis of C. reinhardtii towards HCO3− using an agarose-based microfluidic device. Our device was tested on well-studied chemicals such as NH4Cl and CoCl2 which are chemoattractants and NaCl which is neutral chemical. For the first time, we observed the chemotactic response from C. reinhardtii towards HCO3− and found the concentration with the strongest response. The result of the circadian clock-related chemotaxis assay showed a low chemotaxis response during the daytime, while demonstrated the highest peak in the middle of the night. Furthermore, we verified factors that influence CCM caused a significant change in the chemotactic behavior. This study would provide an essential knowledge on HCO3−induced chemotaxis and promote further understanding of inorganic carbon utilization. |
