| 초록 |
It has been reported that the production of 2,3-butanediol(2,3-BDO) using carbon monoxide as a substrate is commercially viable. However, because of the hydrophilicity of 2,3-BDO, it is not easy to purify it. Therefore, it is necessary to convert 2,3-BDO to other hydrophobic products directly. In this research, for the production of mevalonate using 2,3-BDO as a substrate, recombinant cells were constructed. We transformed plasmid carrying the gene necessary for mevalonate production into Pseudomonas putida KT2440. In the experiments on various carbon sources such as 2,3-BDO, glucose and glycerol, it is confirmed that 2,3-BDO is the most suitable carbon source for mevalonate production. The amount of mevalonate produced under conditions using 2,3-BDO as a carbon source was 1.79 g / L, and the amounts of glucose and glycerol were 0.11 g / L and 0.17 g / L, respectively, for 48 hours. |
