| 학회 |
한국화학공학회 |
| 학술대회 |
2018년 봄 (04/25 ~ 04/27, 창원컨벤션센터) |
| 권호 |
24권 1호, p.394 |
| 발표분야 |
생물화공 |
| 제목 |
Bioconversion of methane to methanol through partial inhibition of methanol dehydrogenase using Methylomonas sp. DH-1 |
| 초록 |
Previous studies have focused on the bioconversion of methane to methanol by supplementing sodium formate as a reducing power source, but sodium formate was too expensive to be used for methanol conversion. In this study, methanol accumulation was achieved only by partial inhibition of methanol dehydrogenase (MDH) and controlling of the NADH pool using whole-cell. To find efficient inhibitors of MDH, five different chemical inhibitors were tested, and EDTA was the most effective to inhibit the activity of MDH. In addition, We determined the optimal EDTA concentration to be 0.5mM because in stoichiometric calculation, about 31% MDH activity should be remained for methanol production. Also, the pH dependent MDH inhibition test was performed. As a result, methanol accumulation was significantly increased in partial inhibition of MDH (6.35mM) compared to that in the absence of MDH inhibitor (0.56mM) and complete inhibition of MDH activity (3.85mM). This notable result demonstrated the feasibility of bioconversion of methane to methanol without using external reducing power regeneration source. |
| 저자 |
한준희, 김유진, 김성일, 김현수, 하선영, 조숙형, 나정걸, 이진원
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| 소속 |
서강대 |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
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