| 초록 |
Silica nanoparticles with specific functions have been spotlighted because of their superior properties such as biocompatibility, low toxicity, etc. However, silica nanoaparticles has disadvantage such as aggregation when they were used in the applications. In this study, we found that the surface modification of silica particles with PVP dramatically increased its dispersibility and stability in water. Silica nanoparticles with Alexa Fluor 647 were coated with PVP and functionalized with aldehyde functional groups. Finally, they were conjugated with specific proteins before applied to LFA chips. We demonstrated that PVP-coated fluorescent silica nanoparticles did not aggregate in assay buffers by analysis of z-potential and TEM. Their fluorescence intensity and photostability were evaluated by photoluminescence (PL) spectroscopy. We successfully applied the pvp-stabilized silica nanoparticles to th LFA detection of troponin I (TnI) as a crardiac marker was measured using a particles. |
