| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Cellular Responses to Nanotopology of Polymeric Surfaces Fabricated with AAO Nanoimprinting |
| 초록 | We made various patterns on the PMMA surfaces and observed the cellular responses on them. Nanoimprinting using AAO (anodized aluminum oxide) template was used for the topology control of polymeric culture surfaces. AAO has numerous regularly-spaced nanopores. The pore diameter and the center-to-center pore distance can be easily controlled by altering the anodizing and etching conditions. One of the main advantages of using AAO as a template in nanoimprinting is that nanometer-scale structure templates larger than 25 cm2 are easily, economically and quickly fabricated, which are suitable for various cellular studies. We cultured fibroblast on the variously nanopatterned surfaces directly or after surface treatment with collagen, O2 plasma, or both. The cellular activities of fibroblasts on 420nm-nodule pattern treated with both were significantly retarded at 24 hours. Fibroblasts also showed significantly different activities among different patterns. In the time-lapse observation of initial adhesion in stressed condition (ambient temperature and no CO2 supply) from 3.5 hours to 5.5 hours after seeding, we could conclude that fibroblast do not like 420nm-nodule pattern for their initial adhesion as fibroblasts on 420nm-nodule pattern markedly changed their shape to round form while those on 90nm-nodule pattern maintained their shape. REFERENCES [1] A. Curtis and C. Wilkinson, "Nantotechniques and approaches in biotechnology," Trends Biotechnol, vol. 19, pp. 97-101, 2001. [2] T. J. Webster, L. S. Schadler, R. W. Siegel, and R. Bizios, "Mechanisms of enhanced osteoblast adhesion on nanophase alumina involve vitronectin," Tissue Eng, vol. 7, pp. 291-301, 2001. [3] R. S. Kane, S. Takayama, E. Ostuni, D. E. Ingber, and G. M. Whitesides, "Patterning proteins and cells using soft lithography," Biomaterials, vol. 20, pp. 2363, 1999. [4] Y. Xia and G. M. Whitesides, SOFT LITHOGRAPHY, vol. 28, 1998. [5] L. J. Guo, "Recent progress in nanoimprint technology and its applications," Journal of Physics D: Applied Physics, pp. R123, 2004. [6] S. Y. Chou, C. Keimel, and J. Gu, "Ultrafast and direct imprint of nanostructures in silicon," Nature, vol. 417, pp. 835, 2002. [7] Y.-L. Loo, R. L. Willett, K. W. Baldwin, and J. A. Rogers, "Interfacial chemistries for nanoscale transfer printing," Journal of the American Chemical Society, vol. 124, pp. 7654, 2002. [8] S. Matsui, Y. Igaku, H. Ishigaki, J. Fujita, M. Ishida, Y. Ochiai, H. Namatsu, and M. Komuro, "Room-temperature nanoimprint and nanotransfer printing using hydrogen silsequioxane," Journal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures, vol. 21, pp. 688, 2003. [9] E. Kim, Y. Xia, and G. M. Whitesides, "Polymer microstructure formed by moulding in capillaries," Nature, vol. 376, pp. 581, 1995. [10] H. Masuda and M. Satoh, "Fabricationj of gold nanodot array using anodic porous alumina as an evaporation mask," Jpn. J. Appl. Phys., vol. 35, pp. L126-L129, 1996. |
| 저자 | 김대현, 이규백 |
| 소속 | 고려대 |
| 키워드 | Nanoimprinting; AAO; nanotopology; nanopattern; cellular resoponse |
