| 학회 |
한국화학공학회 |
| 학술대회 |
2014년 봄 (04/23 ~ 04/25, 창원컨벤션센터) |
| 권호 |
20권 1호, p.419 |
| 발표분야 |
생물화공 |
| 제목 |
Microbial production of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) by metabolic engineering |
| 초록 |
Polyhydroxyalkanoates(PHAs) are polyesters accumulated in many bacteria. Among lots of PHAs, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] is one of the important copolymer because of lower melting point and flexibility compared to P(3HB). So far, for the production of P(3HB-co-3HV), adding of second auxiliary carbon source was needed. However, due to the toxicity of auxiliary carbon source, it is very hard to maintain the balance between cell growth and P(3HB-co-3HV) production. Thus, we developed the E. coli can stably synthesize 3HB-CoA and 3HV-CoA in controlled ratio from glucose without feeding of exogenous auxiliary carbon source by metabolic engineering. This engineered strain can efficiently synthesize P(3HB-co-3HV) independent with exogenous auxiliary carbon source. "This work was supported by the Technology Development Program to Solve Climate Changes from National Research Foundation of Korea (Development of systems metabolic engineering platform technologies for biorefineries; NRF-2012-C1AAA001-2012M1A2A2026556) and Intelligent Synthetic Biology Center (2011-0031963) of Korea through the Global Frontier Research Program of the Ministry of Education, Science and Technology (MEST)." |
| 저자 |
최소영1, 양정은1, 최용준1, 이승환2, 송봉근2, 박시재3, 이상엽1
|
| 소속 |
1KAIST, 2화학(연), 3명지대 |
| 키워드 |
biopolymer; metabolic engineering
|
| E-Mail |
|
| 원문파일 |
초록 보기 |
