| 학회 |
한국고분자학회 |
| 학술대회 |
2010년 가을 (10/07 ~ 10/08, 대구 EXCO) |
| 권호 |
35권 2호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
In vitro Test for the Bone Marrow Stromal Cells with Silk Fibroin Contents in Silk/PLGA Scaffold |
| 초록 |
Silk Fibroin(SF) has been increasingly studied for new biomedical applications due to the biocompatibility, slow degradability and remarkable mechanical properties of the material. We examined the effects of silk fibroin scaffold properties on BMSCs attachment, proliferation and differentiation. We developed the SF loaded poly(L-lactide-co-glycolide)(PLGA) scaffolds. SF/PLGA 0, 10, 20, 40 and 80 wt% scaffolds were prepared by solvent casting/salt leaching method. These cells were seeded in prepared SF/PLGA scaffold. The effect of SF/PLGA scaffold on the proliferation and differentiation of rat BMSCs were assessed in culture using the MTT assay and RT-PCR was conducted to confirm mRNA expression of ALP and Osteocalcin. According to our results, 80% SF/PLGA scaffold have good cell compatibility and attachment was higher. This Research was Supported WCU (R31-20029) and by SCRC (SC4110). |
| 저자 |
김수진, 유현, 정선영, 김형은, 송병주, 강길선
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| 소속 |
전북대 |
| 키워드 |
Bone Marrow Stromal Cell; Silk Fibroin; PLGA; Scaffold; differentiation
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| E-Mail |
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