| 학회 |
한국화학공학회 |
| 학술대회 |
2006년 가을 (10/27 ~ 10/28, 고려대학교) |
| 권호 |
12권 2호, p.1803 |
| 발표분야 |
생물화공 |
| 제목 |
An enzyme-nanofiber composite for enzyme stabilization |
| 초록 |
We constructed an enzyme-nanofiber composite in which enzyme was stable. This study will show the preparation and application of enzyme-nanofiber composites. The enzyme-nanofiber composite was prepared by coating enzyme-aggregate on the surface of nanofibers. The esterase from Rhizopus oryzae was used to construct this biocatalytic nanofibers. The activity and stability of the enzyme-nanofiber composite was measured by using 4-nitrophenyl butyrate as substrate dissolved in N,N-Dimethylformamide (DMF). It was found that immobilized enzyme on nanofibers was highly stable even under shaking condition, preserving 80 % of the initial activity for 80 days. Repeated usages of this enzyme-nanofiber composites seem to be very promise. The enzyme-nanofiber composite was repeatedly used for 30 cycles for enzymatic substrate hydrolysis and the immobilized enzyme still remained to be highly active even after 30 cycles. The enzyme-nanofiber composite allowed an economically feasible enzyme system for costly enzymes with potential applications in food production, pharmaceuticals application and bioremediation process. |
| 저자 |
이진형1, 황이택2, 김병찬1, 김중배3, 상병인4, 구만복2
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| 소속 |
1광주과학기술원 환경모니터링센터, 2고려대, 3Pacific Northwest National Laboratory (PNNL), 4한국과학기술원 유해물질연구센터 |
| 키워드 |
enzyme stabilization; nanofiber; esterase; enzyme immobilization
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| E-Mail |
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| 원문파일 |
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