| 학회 |
한국화학공학회 |
| 학술대회 |
2007년 봄 (04/19 ~ 04/20, 울산 롯데호텔) |
| 권호 |
13권 1호, p.13 |
| 발표분야 |
고분자 |
| 제목 |
A Label-Free Immunosensor Chip Based on Polydiacetylene Vesicle Arrays for Bacteria Detection |
| 초록 |
The conjugated polydiacetylene (PDA) vesicles have been proved as convenient chemical and biological sensors due to their unique chromatic properties. The diacetylene lipids, undergo polymerization via 1,4-addition reaction upon UV light to form an ene-yne alternating polymer chains, producing liposome-like vesicles in aqueous solution. The polymerized vesicles have a bichromic property from blue to red upon external perturbations. Here we developed antibody-affixed PDA sensors on a solid substrate as an array form. An antibody for detecting Esherichia coli was covalently linked to the PDA vesicles. Although antibody-antigen binding plays a key role in many medical diagnoses through various techniques, the attempts to develop biosensors using antibody affixed PDA vesicles have been scarce. In this work, we demonstrate antibody-affixed PDA vesicles on the solid substrates as a robust chip-type biosensor which can detect bacteria, complicated biological analytes, without any labeling procedure. |
| 저자 |
최 현1, 김경우1, 오민규1, 안동준1, 이길선1, 김종만2
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| 소속 |
1고려대, 2한양대 |
| 키워드 |
polydiacetylene; E.coli detection; cell chip
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| E-Mail |
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| 원문파일 |
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