| 학회 |
한국화학공학회 |
| 학술대회 |
2015년 가을 (10/21 ~ 10/23, 일산 KINTEX) |
| 권호 |
21권 2호, p.1736 |
| 발표분야 |
생물화공 |
| 제목 |
Metabolic engineering of Corynebacterium glutamicum to overproduce L-ornithine |
| 초록 |
L-Ornithine is a non-essential amino acid with various applications in food industry. We aim at high-titer production of L-ornithine by Corynebacterium glutamicum ATCC 13032 through metabolic engineering. In addition to deletions of proB and argF, the argR gene encoding the regulatory repressor of L-arginine operon was also deleted to enhance the ornithine flux. Furthermore, plasmid-based overexpression of argCJBD genes, changing the start codons of pgi and zwf and replacing the native promoter of the tkt operon with the strong sod promoter resulted in a titer of 51.5 g/L of L-ornithine with productivity of 1.29 g/L/h under fed-batch conditions. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF) of Korea (NRF-2012-C1AAA001-2012M1A2A2026556).] |
| 저자 |
이상엽1, Joungmin Lee2, Seo Yun Kim3, Ziwei Luo4
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| 소속 |
1MBEL, 2Dept. of Chemical and Biomolecular Engineering (BK21 Plus rogram), 3KAIST / BioInformatics Research Center, 4Institute for the BioCentury |
| 키워드 |
L-ornithine; Corynebacterium glutamicum; Metabolic engineering
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| E-Mail |
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| 원문파일 |
초록 보기 |
