| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.274 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Chitosan Microspheres for a Nasal Vaccine Delivery System in vitro and in vivo |
| 초록 | Chitosan is a valuable excipient for nasal drug delivery system due to its biocompatibility, biodegradability and low cost. Also, it has been reported that chitosan could induce cytotoxic T lymphocytes and macrophage activation. Chitosan microspheres (CMs) were prepared based on ionic gelation process of tripolyphosphate and then, the microspheres were loaded with Bordetella Bronchiseptica Dermonecrotoxin (BBD), a virulence factor of a causative agent of atrophic rhinitis, for nasal vaccination. Particle sizes of BBD-loaded CMs were smaller than 10 μm and had narrow size distribution with uniformity. Vaccine BBD release from BBD-loaded microspheres and secretion of cytokine such as tumor necrosis factor-α (TNFα) and nitric oxide (NO) from BBD-loaded CMs were checked in vitro (Fig. 1). Also, BBD-loaded CMs were treated to mouse as a model animal, and systemic and nasal immunities were checked in vivo (Fig. 2). Fig. 1. Secretion of TNF-α from murine macrophage stimulated with BBD, BBD-loaded CMs, LPS and control (n=3). Fig. 2. Level of anti-BBD IgA of mouse after nasal wash with BBD-loaded CMs. |
| 저자 | 강호림1, 박인규1, 강상균2, 강미란2, 유한상2, 조종수1 |
| 소속 | 1서울대 농생명과학대, 2서울대 수의과대 |
| 키워드 | chitosan microspheres; nasal vaccination |
