| 학회 |
한국고분자학회 |
| 학술대회 |
2014년 가을 (10/06 ~ 10/08, 제주 ICC) |
| 권호 |
39권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
The synergetic action of co-delivered gene and peptide with targeting peptide-grafted LMWSC in cancer cells. |
| 초록 |
The aim of this study was to deliver gene and peptide for synergetic action against tumor. The ternary complexes of gene, peptide, and targeting peptide-conjugated LMWSC were prepared by electrostatic interaction in aqueous solution. The binding affinity, pDNA release, and stability from DNase of ternary complexes were confirmed by electrophoresis. The particle size and zeta potential of ternary complexes was evaluated by DLS. The result of tranfection assay showed that ternary complexes with targeting peptide-conjugated LMWSC was more effective than that without peptide for target at cancer cell. The cytotoxicity and antitumor activity of ternary complexes were confirmed by MTT assay. These results suggest that co-delivered gene and peptide can effectively inhibit tumor growth. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education. Science and Technology (NRF-2012R1A2A2A01014898). |
| 저자 |
정경원, 김태훈, 박성철, 박영훈, 장미경, 나재운
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| 소속 |
순천대 |
| 키워드 |
gene; peptide; targeting peptide conjugated-LMWSC
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| E-Mail |
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