화학공학소재연구정보센터
학회 한국화학공학회
학술대회 2014년 봄 (04/23 ~ 04/25, 창원컨벤션센터)
권호 20권 1호, p.351
발표분야 생물화공
제목 Cell-free enzyme system for bio-ethanol production
초록 Over the last few decades, bio-ethanol has emerged the most important alternative energy resource. Cell-free fermentation: a non-cellular approach is among the endeavors in biotechnology today providing a solution for the complexities and limitations linked with conventional fermentation. We developed the cell-free enzyme system through beads beating and insured the presence of enzymes and cofactors through LC-MS/MS Q-TOF and assay kit, respectively. The system was comprised of complete machinery required for the cascade of the biological reactions involved in microbial fermentation. The most important feature of the system was the shifting of ethanol production towards higher temperature (<40°C) than conventional fermentation temperature. At elevated temperature (40°C) the system was found more effective (3.83 g/L) than conventional fermentation system (0.48 g/L). This provided an insight for the development of simultaneous saccharification and fermentation process via cell-free enzyme system.
저자 Waleed Ahmad Khattak, Mazhar-ul-Islam, Muhammad Wajid Ullah, Shaukat Khan, 유보완, 김민아, 김예지, 박중곤
소속 경북대
키워드 Non-cellular fermentation; bioethanol; conventional fermentation; beads beating
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