| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO)) |
| 권호 |
23권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Fine-tuning of gapA expression in Escherichia coli for the optimization of isoprenoids production |
| 초록 |
Here, we demonstrate that precursor balancing is one of the critical steps for the production of isoprenoids in Escherichia coli. First, the implementation of the synthetic lycopene production pathway as a model system and the amplification of the native DXP pathway were accomplished using synthetic constitutive promoters and redesigned 5′-untranslated regions (5′-UTRs). Next, fine-controlled precursor balancing was investigated by tuning phosphoenolpyruvate synthase (PpsA) or glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The results showed that tuning-down of gapA improved the specific lycopene content by 45% compared to the overexpression of ppsA. The specific lycopene content in the strains with down-regulated gapA increased by 97% compared to that in the parental strain. Our results indicate that gapA is the best target for precursor balancing to increase biosynthesis of isoprenoids. |
| 저자 |
김성경1, 정주영1, 임재형1, 김세연1, 임대균2, 석주연1, 이승재1, 정규열1
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| 소속 |
1포항공과대, 2고려대 |
| 키워드 |
precursor balancing; lycopene; gapA; ppsA; G3P
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| E-Mail |
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