| 초록 |
Cyanobacteria have been gathering global interests regarding CO2 bio-mitigation due to the ability to direct utilization of CO2. Engineered cyanobacteria have been enabled to produce industry-relevant chemicals from CO2. However, the final products have often been mixed with fermented metabolites during dark fermentation. Our engineering of Synechococcus elongatus PCC 7942 enabled continuous conversion of CO2 to acetone as sole product via both ATP-driven malonyl-CoA synthesis pathway and heterologous phosphoketolase-phosphotransacetylase pathway. Because of strong correlations between the metabolic pathways of acetate and acetone, supplying the acetyl-coA directly from CO2 in the engineered strain, led to sole production of acetone without changing nutritional constraints, and without an anaerobic shift. Subsequently, 5% (v/v) CO2 gas-stripping-based recovery systems allowed collection of the sole production of acetone from CO2. Our engineered strains could be modified to create bio-solar cell factories for sustainable photosynthetic production of acetyl-CoA-derived biochemicals. This work was supported by NRF (University-Institute cooperation Program, 2015). |
