| 학회 | 한국화학공학회 |
| 학술대회 | 2011년 가을 (10/26 ~ 10/28, 송도컨벤시아) |
| 권호 | 17권 2호, p.1865 |
| 발표분야 | 재료 |
| 제목 | Enzyme activity assay of horseradish peroxidase encapsulated in peptide nanotubes |
| 초록 | The objectives of this study are to demonstrate the encapsulation of horseradish peroxidase (HRP) inside a peptide nanotube (PNT) and to measure the activity of the encapsulated enzyme. The bulk enzymes of 0.15 μg were observed to be encapsulated into 1 mg of PNTs by enzyme assay. The encapsulation was also verified with TEM. The enzyme activity of HRP encapsulated inside PNTs was examined in terms of thermal stability, long-term stability, and resistance to a denaturant. The enzyme assay results showed that the encapsulated HRP had good storage stability and retained its activity up to 90% for 18 days while the free HRP in the solution lost about 50% activity during the same period. At a raised temperature of 55 °C, the enzyme activity of HRP in PNTs stayed constant at 20% higher than that of the free HRP. In the presence of the denaturant such as guanidine hydrochloride (GmHCl), the activity of the encapsulated HRP was maintained around 10% higher than the activity of the free HRP. This work was supported by NANO-Star center under the CRADA of AFIT-UT-KWU. |
| 저자 | 김태용1, 박병욱2, 김동식2, 윤도영1 |
| 소속 | 1광운대, 2Univ. of Toledo |
| 키워드 | Enzyme activity; peptide nanotube |
| 원문파일 | 초록 보기 |
