| 학회 |
한국공업화학회 |
| 학술대회 |
2007년 가을 (11/02 ~ 11/03, 한경대학교) |
| 권호 |
11권 2호 |
| 발표분야 |
생물공학 |
| 제목 |
Effect of Acyl-CoA’s on the Production of Doxorubicin in Streptomyces peucetius ATCC 27952 |
| 초록 |
An anticancer agent doxorubicin biosynthesis is initiated from one propionyl-CoA starter unit and nine malonyl-CoA extender units in S. peucetius. We increased the acyl-CoA pools by integrating E. coli pantothenate kinase gene into S. peucetius to produce more doxorubicin. Pantothenate kinase is the key regulatory enzyme in CoA biosynthetic pathway. As a result, ε-rhodomycinone, a major aglycon intermediate of doxorubicin, production was enhanced. To produce more doxorubicin from this ε-rhodomycinone, glycosyltransferase or glycon-biosynthetic genes were overexpressed in panK-integrated S. peucetius. Major metabolites from these strains were quantified and compared. |
| 저자 |
송은정1, Sailesh Malla2, 이광원1, 송재경2, 김병기1
|
| 소속 |
1서울대, 2선문대 |
| 키워드 |
Streptomyces peucetius; pantothenate kinase; doxorubicin; coenzyme A
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| E-Mail |
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