| 학회 | 한국화학공학회 |
| 학술대회 | 2003년 봄 (04/25 ~ 04/26, 순천대학교) |
| 권호 | 9권 1호, p.515 |
| 발표분야 | 생물화공 |
| 제목 | Toxicity classification using recombinant bioluminescent bacteria with katG, sodA and pqi promoter in the a multi-channel continuous monitoring system |
| 초록 | A multi-channel system was developed for continuous toxicity monitoring and classification of toxicity. This system consists of a series of parallel two-stage minibioreactors, in which the recombinant bioluminescent bacterial cells were growing actively in the first stage minibioreactors, while the fed cells from the first reactors were mixed with toxic samples in the second minibioreactors. Each channel was used for cultivating different recombinant bacterial strains, such as EBHJ2 (sodA::luxCDABE), EBJM1 (pqi-5::luxCDABE) and DK1 (katG::luxCDABE). EBHJ2 can be able to detect contaminants which cause oxidative damage or unbalance through increases in ROS. DK1 is sensitive to H2O2. EBJM1 has shown sensitive response to paraquat, a superoxide radical-generating agent. By using different bioluminescent strains, the ability of the multi-channel system to classify toxicity can be enhanced. |
| 저자 | 이진형, 김병찬, 안주명, Robert J. Mitchell, 구만복 |
| 소속 | 광주과학기술원(K-JIST) NRL on Environmental Biotechnology |
| 키워드 | recombinant bacteria; biosensor; katG; sodA; pqi; multi-channel continuous system |
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