| 초록 |
The fermentative production of 2,3-butanediol has been intensively studied because of the potential of these products as platform chemicals, such as polymers, fuels, and plastics. Klebsiella pneumoniae is a good host for production of 2,3-butanediol from a wide range of carbon sources. We metabolically engineered K. pneumoniae (△wabG △ldhA △pflB) to reduce the production of byproducts. In particular, the inactivation of pflB significantly improved the yield of 2,3-butanediol from glucose to 92.2% of the theoretical maximum. We also engineered Enterobacter aerogenes for efficient utilization of sugarcane molasses in 2,3-butanediol production by elimination of carbon catabolite repression. Deletion of scrR and cra as well as overexpression of scrAB improved the strain’s performance of utilizing three sugars in sugarcane molasses and producing 2,3-butanediol. |
