| 학회 | 한국공업화학회 |
| 학술대회 | 2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 | 25권 1호 |
| 발표분야 | 포스터-생물공학 |
| 제목 | The reversal effect of hypoxic condition for cell via Oxygen nanobubble(ONB) |
| 초록 | When cancer develops, the surrounding tissues become deficient in oxygen supply. Hypoxia increases the expression of HIF-1a due to a lack of oxygen supply. Thus, a few membrane proteins act as an efflux pump that release the drugs out of the cell. It lowers the drug efficiency. So we fabricated an oxygen nanobubble(ONB) consisting of a phospholipid shell and an oxygen core to reverse hypoxia. Cellular morphology and oxygen distribution were changed in hypoxia. Cells under hypoxic condition didn’t grow well and became small-sized spherical shape. We fabricated ONB and confirmed ONB has no toxicity through the cell cytotoxicity test. The effect of ONB to helps reverse the hypoxia was confirmed by image-IT hypoxia reagent and live/dead assay. With ONB treatment, the number of hypoxia cells were decreased. And increased expression of HIF-1a in hypoxia cells was confirmed using ELISA. It is expected that ONB can be used for oxygen delivery to cells in the core of spheroid and organoid. |
| 저자 | 홍주혜, 최종훈 |
| 소속 | 중앙대 |
| 키워드 | hypoxia cell; HIF-1a; oxygen delivery; reverse hypoxia |
