| 초록 |
Isoprenoids form one of the most diverse group of natural products. Biosynthesis of isoprenoids via DXP pathway requires equimolar glyceraldehyde 3 phosphate and pyruvate. In this study, we explored efficient engineering for precursor balancing and demonstrated that the importance of precise precursor balancing to divert carbon flux toward isoprenoids in E. coli. First, we constructed a lycopene-overproducing E. coli strain by the amplification of the native DXP pathway. Second, we investigated the effect of precise precursor balancing with PpsA and GAPDH through fine-tunable control at both transcription and translation levels. The results showed that the specific lycopene content in optimal gapA variants increased up to 97% compared to that of the parental strain. Finally, our strategy could be broadly utilized in the field of metabolic engineering and synthetic biology to increase the production of numerous high-value isoprenoids. |
