| 학회 |
한국공업화학회 |
| 학술대회 |
2020년 가을 (10/28 ~ 10/30, 광주 김대중컨벤션센터(Kimdaejung Convention Center)) |
| 권호 |
24권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Precise precursor balancing for the biosynthesis of isoprenoids production through fine control of gapA expression in Escherichia coli |
| 초록 |
Isoprenoids form one of the most diverse group of natural products. Biosynthesis of isoprenoids via the DXP pathway requires equimolar glyceraldehyde 3 phosphate and pyruvate to divert carbon flux toward the products of interest. In this study, we demonstrated that precursor balancing is one of the most crucial steps for diverting carbon flux toward isoprenoids via the DXP pathway in E. coli. First, we constructed a lycopene-overproducing E. coli strain by the amplification of the DXP pathway using synthetic promoters and redesigned 5’-UTR. Next, fine-controlled precursor balancing was investigated by tuning PpsA or GAPDH. The results showed that tuning-down of gapA improved the specific lycopene by 45% compared to the overexpression of ppsA. The specific lycopene content in the strains with down-regulated gapA increased by 97% compared to that in the parental strain. Our results indicate that gapA is the best target for precursor balancing to increase biosynthesis of isoprenoids |
| 저자 |
정금영, 정주영, 임재형, 정규열
|
| 소속 |
포항공과대 |
| 키워드 |
fine control; isoprenoid; precursor balancing; gapA; ppsA; G3P
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| E-Mail |
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