| 학회 | 한국공업화학회 |
| 학술대회 | 2020년 가을 (10/28 ~ 10/30, 광주 김대중컨벤션센터(Kimdaejung Convention Center)) |
| 권호 | 24권 1호 |
| 발표분야 | 포스터-생물공학 |
| 제목 | Oxygen nanobubbles(ONB) for the reversal of hypoxic cells in microenvironments |
| 초록 | When cancer develops, tissues are deficient in oxygen. Hypoxia increases resistance to drugs, making treatment difficult. We fabricated an ONB consisting of a phospholipid shell and an oxygen core. it is non-toxic to cells and helps reverse the hypoxia. Cellular morphologies and oxygen distribution was changed by the hypoxia in different cells(e.g. cancer cells, normal cells, and macrophages). Cells grown under hypoxia conditions didn’t die but did not grow well either, and they were spherical in shape. This was confirmed by microscopy and live/dead assay. The oxygen distribution was confirmed by image-IT hypoxia reagent. Cells also express HIF-1a due to a lack of oxygen. Increased expression of HIF-1a in hypoxia cells was confirmed using ELISA. When ONB is treated on these hypoxia cells. The cells begin to grow again, and the oxygen distribution increases. It is expected that oxygen nanobubbles can be used for oxygen transfer of cells in the production of spheroids and organoids. |
| 저자 | 윤세미, 최종훈 |
| 소속 | 중앙대 |
| 키워드 | hypoxia; cell; oxygen; nanobubble |
