| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Mechano-active cartilage tissue engineering using a highly elastic scaffold and dynamic stimualation |
| 초록 | Introduction It is known that complex loading is concerned in the development and maintenance of articular cartilage in the body. It means the compressive mechanical stimulation is a very important factor for formation of articular cartilage using a tissue engineering technique. The objective of this study is to engineer cartilaginous constructs with a mechano-active scaffold and to evaluate the effect of dynamic compression for regeneration of cartilage. Materials and Methods A highly elastic scaffold was fabricated from very elastic poly(L-lactide-co-carprolactone)(5:5) with 85 % porosity and 300~500 μm pore size by a gel-pressing method. The scaffolds were seeded with chondrocytes and the continuous compressive deformation was applied to them with 0.1Hz for 10 days and 24 days respectively to evaluate the effect of dynamic compression for regeneration of cartilage. Also, the chondrocytes-seeded constructs were implanted in nude mice subcutaneously to investigate their biocompatibility and cartilage formation. Results and Discussion Mechano-active scaffolds whose tensile strain at break was more than 500% were prepared. Collagen and GAG contents of cell-polymer constructs showed that chondrogenic differentiation was sustained and enhanced significantly and chondrial extracellular matrix was increased through mechanical stimulation. Histological analysis of the specimens retrieved at 8 weeks with hematoxylin and eosin (H&E) staining revealed implants stimulated mechanically by compression formed mature and well-developed cartilaginous tissues, as evidenced by chondrocytes within lacunae. Safranin O and Alcian blue staining indicated an abundant accumulation of GAGs in implants stimulated mechanically. : The periodic application of dynamic compression can encourage chondrocytes to maintain their phenotypes and enhance GAGs production and consequently, improve the quality of cartilaginous tissue formed in vitro and in vivo. Figure 1. Histological studies of implants 8 weeks after implantation (Implants of the upper layer were stimulated on a compressive bioreactor before implantation for 10 days and implants of the lower layer were cultivated at static condition for 10 days). Acknowledgements This work was supported by Korea Ministry of Science and Technology, M6-0302-00-0017. References 1. O. Demarteau, et. al., Biochem Biophy Res Comm 2003;310:580-588 2. 3. Eric M. Darling, et. Al., Annu Biomed Eng 2000;2:1114-1124 |
| 저자 | 정영미1, 김수현1, 김상헌1, 김영하2, Jun Xie3, Takehisa Matsuda4, 민병구3 |
| 소속 | 1한국과학기술(연), 2광주과학기술원, 3Department of Biomedical Engineering, 4Kyushu Univ. |
| 키워드 | a elastic scaffold; dynamic stimulation; tissue engineered cartilage |
