| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.460 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Mechano-active vascular tissue engineering using very elastic PLCL scaffolds |
| 초록 | Many recent studies have reported that mechanical stimuli enhanced the development and function of engineered vessel tissues1. For such stimuli-enhanced (mechano- active) tissue engineering, it is necessary to develop very elastic scaffolds to deliver cyclic mechanical strain without significant permanent deformation. We applied very elastic poly(lactide-co-caprolactone) (PLCL) scaffolds for mechano-active vascular tissue engineering2 to culture VSMCs (vascular smooth muscle cells) and ECs (enthothelial cells) on a pulsatile-flow bioreactor3 to regenerate native-like vascular tissues. Tubular PLCL scaffolds demonstrated a complete resilience under cyclic strain even in culture medium for the initial 2 weeks. The cell number in the engineered tissues continuously increased for up to 5 weeks in both static and pulsatile flow culture groups. The regenerated grafts showed well developed SM tissues within the scaffolds(그림 1). Collagen and elastin production was enhanced for pulsatile flow group compared with static culture group (그림 2). The collagen or elastin contents reached about the half values of native vessels. From the Western blotting analysis, the phenotypes of SMC were better maintained for pulsatile flow group while those for static culture group were slowly decaded. Burst strength of engineered vessels was increased on culture to reach the values of native vessels of rabbit arota. An implantation test using animal models of regenerated vessels is in plan.SMCs were seeded onto very elastic PLCL scaffolds and cultured on a pulsatile perfusion reactor (mechano-active tissue engineering). A mechanical stimulation encouraged SMCs to maintain their phenotypes and enhanced elastin production. The rubber-like elasticity of PLCL applied in this study might have contributed to transfer the mechanical signal to cultured cells completely to reveal the effect of mechanical stimuli on tissue engineering. The regenerated vessels exhibited the burst strength similar to native vessels. Fig. 1. H&E staining of vascular constructs tissue ngineered under dynamic stimulation (P=Polymer) Fig. 2. Elastin contents of vascular constructs tissue engineered under dynamic stimulation 1. L.E.Niklason, R.Langer, et al. Science, 284, 489 (1999). 2. S.I.Jeong, S.H.Kim, Y.H.Kim, et al, J. Biomat. Sci., Polymer Ed., 15, 645-60 (2004). 3. S.I.Jeong, J.H.Kwon, J.I.Lim, S.H.Kim, Y.H.Kim, et al., Biomaterials, 26, 1405-11 (2005) |
| 저자 | 임진익1, 권재현1, 성원준1, 정민섭1, 김상헌1, 김수현1, 김수자2, 김영하1 |
| 소속 | 1한국과학기술(연), 2경희대 |
| 키워드 | tissue engineering; bioreactor; vascular graft; scaffold |
