| 학회 |
한국공업화학회 |
| 학술대회 |
2016년 가을 (10/26 ~ 10/28, 제주국제컨벤션센터(ICCJEJU)) |
| 권호 |
20권 2호 |
| 발표분야 |
(석유화학윤활유) C1 리파이너를 위한 화학 및 바이오 공정 개발 |
| 제목 |
Cell-free synthesis and analysis of enzymes having industrial implications |
| 초록 |
While microbial cells have widely been used for the production of recombinant enzymes, the use of living cells sets intrinsic limitations in numerous dimensions including the throughput of process and choice of assay substrates. In addition, intracellullar conditions are often incompatible with proper expression and folding of target proteins. These limitations associated with cell-based expression can be effectively overcome by employing cell-free protein synthesis as an alternative expression platform. Not being subject to the constraints of maintaining cell viability and intact cell membranes, cell-free protein synthesis offers greater flexibility in designing expression and screening procedures. In this study, we will discuss different approaches to harness the advantages of cell-free synthesis for efficient production of enzymes that have industrial implications in different sectors of industrial biotechnology. |
| 저자 |
김동명 |
| 소속 |
충남대 |
| 키워드 |
industrial enzymes; expression; cell-free protein synthesis
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| E-Mail |
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