| 초록 |
Adult articular cartilage tissue has poor capability of self-repair. Therefore, a variety of tissue engineering approaches are motivated by the clinical need for articular repair. 0, 0.1, 0.5 and 1% DBP/Alginate microcapsules were fabricated by seeded chondrocytes into microcapsules. After 1, 5, 7, 10 and 14 days of cell seeding, cell proliferation activity was measured via MTT assay. Morphological observation, histology, biological assay for collagen type I, II and aggrecan, and PCR were performed at each time point of 1, 2 and 3 weeks. In the result, cell viability was higher 1% DBP/alginate microcapsules than the other DBP/alginate microcapsules. DMMB results shown that the highest content of glycosaminoglycan (GAGs) and collagen at 1% DBP/alginate microcapsules. This study suggests that 1% DBP/alginate microcapsules may serve as a potential cell delivery vehicle and a structural basis for tissue engineered articular cartilage. This research was supported by WCU (R31-20029). |
