| 초록 |
We developed simple, and fast assay based Alkaline Phosphatase (ALP)-sensitive sensor via colorimetric change of fluorescent polymer modified surface for detection and antimicrobial activity. This cationic polymer [C/C12-PVP], obtained from quaternization of catechol and bromododecane with poly(vinylpyrrolidone) conjugated with 2-hydroxychalcone (HCAP) to produce (HCAP-PVP). Under treatments with bacteria, ALP presence caused cleavage of phosphate group in HCAP, making color change from greenish-yellow to deep-red. The HCAP-PVP was coated onto polypropylene (PP) films to perform solid-phase bacterial detection, showing similar colorimetric behavior with aqueous state. This platform also showed antimicrobial properties due to presence of quaternary ammonium on dodecane backbone. Therefore, this system can be used as enzymatic-sensitive bacteria platform in solid state to detect and kill bacteria. This work was supported by Korea National University of Transportation in 2018. |
