| 학회 | 한국공업화학회 |
| 학술대회 | 2015년 봄 (04/29 ~ 05/01, BEXCO (부산)) |
| 권호 | 19권 1호 |
| 발표분야 | 생물공학_포스터 |
| 제목 | Micro arrayed polydiacetylene liposome biosensor using gold nanoparticles for Signal enhancement |
| 초록 | Polydiacetylene (PDA) liposomes which possess unique properties and that properties allow liposomes to change color and emit fluorescence in response to stimulus such as temperature, antibody-antigen interaction, variations in pH, mechanical stress and organic solvent. This material has been extensively studied as signal transducers in biosensor applications. In this research, we demonstrated an antibody-based biosensor using PDA liposomes for detection of hIgE. Target hIgE chemically bound to antibodies-immobilized PDA liposomes and the fluorescence responses were a little bit increased depending on target protein concentration. On the other hand, in case of the addition of gold nano particles-conjugated polyclonal antibody on the liposomes, fluorescent responses dramatically increased depending on target protein concentration. As the results, we could confirm that gold nano particles-conjugated polyclonal antibody affect fluorescent signal amplification of PDA liposomes chip. This strategy can be useful to detect proteins of ultra low concentration. |
| 저자 | 김우현1, 원상호2, 심상준1 |
| 소속 | 1고려대, 2그린스쿨 |
| 키워드 | PDA; Polydiacetylene; biosensor |
