| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Development of selection-free stable and tunable gene expression system with minimized cell-to-cell variation |
| 초록 |
Although benefits of multi-copy expression of the genes, plasmids require antibiotics for stable maintenance. Here, we developed the antibiotic-free auxotrophic plasmid maintenance system for stable and tunable gene expression by precise control of plasmid copy number (PCN) in Escherichia coli. We expressed essential gene, infA encoding translation initiation factor, on plasmid instead of chromosome. With this system, the gene expression was stably maintained for 40 generations with minimized cell-to-cell variation. Moreover, the PCN was rationally tunable in 5.6-fold by varying expression of infA. This system was applied for engineering E. coli to produce itaconic acid and lycopene. Notably, the PCN control enabled significant improvement on the production of both itaconic acid and lycopene (2-fold) compared to that of the system based on antibiotics. Collectively, the developed strategy could be a platform for production of value-added products in antibiotic-free cultivation. |
| 저자 |
강채원1, 정규열1, 임현규1, 양진아2, 서상우2
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| 소속 |
1포항공과대, 2서울대 |
| 키워드 |
Synthetic auxotrophy; Plasmid copy number; Expression control; Antibiotic-free; Stable expression; Plasmid stability
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| E-Mail |
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