| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO)) |
| 권호 |
23권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Stable and tunable maintenance of multi-copy plasmid by antibiotics-free auxotrophic control |
| 초록 |
Although benefits of multi-copy expression of the genes, plasmids require addition of antibiotics for their stable maintenance despite of economic and environmental problems. Here, we developed the antibiotic-free plasmid maintenance system for stable and tunable gene expression by precise control of plasmid copy number (PCN) in Escherichia coli. To achieve this, we devised a system that expresses essential genes, infA encoding translation initiation factor (IF-1), on plasmid instead of chromosome. With this system, the gene expression was stably maintained for 40 generations with minimized cell-to-cell variation. Moreover, the PCN was rationally tunable in 5.6-fold by varying expression of infA. Notably, the PCN control enabled significant improvement on the production of both itaconic acid and lycopene (2-fold) compared to that of the conventional system. Collectively, the developed strategy could be a platform for production of value-added products in antibiotic-free cultivation. |
| 저자 |
강채원1, 임현규1, 양진아2, 노명현1, 서상우2, 정규열1
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| 소속 |
1포항공과대, 2서울대 |
| 키워드 |
Plasmid; PCN maintenance; PCN regulation; Essential gene expression level
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| E-Mail |
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