| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 가을 (10/31 ~ 11/02, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
22권 2호 |
| 발표분야 |
우수논문(구두)_박사과정 |
| 제목 |
Selection-free tunable gene expression for stable biochemical production with minimized cell-to-cell variation |
| 초록 |
Plasmid has been utilized for microbial biorefinery to overexpress heterogeneous genes with antibiotic selection. Unfortunately, antibiotics usage has drawbacks such as expensive price and heterogeneity of bacterial populations. Here, we developed Stable and Tunable Plasmid (STAPL) system to maintain plasmid without the addition of antibiotics based on essential gene auxotrophy. The STAPL system expresses one of essential genes, infA encoding translation initiation factor, on plasmid instead of chromosome. With this system, more than 97% of plasmids were stably and uniformly maintained for 40 generations. Furthermore, the plasmid copy number (PCN) was controlled in 5.6-folds by variations of expression level of essential gene. The STAPL system was applied to produce itaconic acid and lycopene; the cells robustly produced the desired products without the addition of antibiotics. The STAPL system can be applicable to industrial biochemical production for improved economic feasibility. |
| 저자 |
강채원1, 정규열1, 임현규1, 양진아2, 노명현1, 서상우2
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| 소속 |
1포항공과대, 2서울대 |
| 키워드 |
Synthetic auxotrophy; Plasmid copy number; Expression control; Antibiotic-free; Stable expression; Plasmid stability
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| E-Mail |
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