| 초록 |
π-conjugate polymers, P3MT (poly-(3-methylthiophene)) have been of great interest as chemical/biological sensors due to their capability of signal propagation through the π-conjugation path. We prepared DBSA (dodecylbenzene sulfonic acid) doped P3MT through an anodic alumina nanoporous template. The doped P3MT was functionalized with the aptamer through electrostatic interaction between the sulfuric trioxide (SO3-) of the doped polymer and the amine group (NH+) of the aptamer. The aptamer functionalized P3MTs were spotted on a glass substrate and dried to eliminate solvents. Then proteins was reacted with the spotted P3MT only for 30 min. It was clear that when the proteins were matched to the specific aptamers, photoluminescence (PL) intensity was increased about 30 times than only aptamer attached P3MT. In this study, we detected three proteins (thrombin, PDGF-BB, VEGF) specifically. This new strategy can be applied to chemical/biological sensors based on P3MT. |
