| 학회 |
한국공업화학회 |
| 학술대회 |
2013년 가을 (10/30 ~ 11/01, 대전컨벤션센터) |
| 권호 |
17권 2호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
3-Hydroxyisobutyrate Dehydrogenase-I from Pseudomonas denitrificans ATCC 13867 Degrades 3-Hydroxypropionic Acid |
| 초록 |
This study examined the role and physiological relevance of 3-hydroxyisobutyrate dehydrogenase-I (3HIBDH-I) of Pseudomonas denitrificans ATCC 13867 in the degradation of 3-hydroxypropionic acid (3-HP) during 3-HP production. The gene encoding 3HIBDH-I of P. denitrificans was cloned, expressed and purified in Escherichia coli BL21. The recombinant 3HIBDH-I showed a high catalytic constant (kcat/Km) on (S)-3-hydroxyisobutyrate, but no detectable activity on (R)-3-hydroxyisobutyrate. The kcat/Km determined for 3-HP was 15.40±1.43 mM-1S-1 in the presence of NAD+ at 37 °C and pH 9.0. In addition to (S)-3-hydroxyisobutyrate and 3-HP, 3HIBDH-I utilized L-serine, methyl-D,L-serine, and methyl-(S)-(+)-3-hydroxy-2-methylpropionate. This study revealed the characteristics of 3HIBDH-I and its significance in 3-HP degradation. |
| 저자 |
주생방1, 박성훈1, Philah Lee1, Subramanian Mohan Raj2, Somasundar Ashok3
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| 소속 |
1부산대, 2Centre for Research and Development, 3PRIST Univ. |
| 키워드 |
3-hydroxyisobutyrate dehydrogenase; 3-hydroxyisobutyric acid; 3-hydroxypropionic acid; 3-hydroxypropionate degradation; Pseudomonas denitrificans
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| E-Mail |
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